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Image Search Results
Journal: Cell Death and Differentiation
Article Title: Kidins220/ARMS controls astrocyte calcium signaling and neuron–astrocyte communication
doi: 10.1038/s41418-019-0431-5
Figure Lengend Snippet: Kidins220 expression in astrocytes is required for proper neuronal development. a Wild-type neurons were plated on confluent wild-type (+/+) or Kidins220−/− astrocyte cultures, fixed and stained with anti-β tubulin III antibodies after 3 DIV to visualize, and quantify network development. Left: representative images of neuron–astrocyte cocultures. Scale bar, 25 µm. Middle: Sholl analysis of wild-type neurons grown on either wild-type or Kidins220−/− astrocytes for 3 DIV. Genotype effect: p = 0.03; **p < 0.01, repeated measures ANOVA followed by the Bonferroni’s multiple comparison test (n = 5 for both wild-type and Kidins220−/− cultures). Right: total dendritic length of wild-type neurons grown on either wild-type or Kidins220−/− astrocytes for 3 DIV. **p < 0.01, unpaired Student’s t test (n = 22 wild-type and Kidins220−/− cells from five independent cultures). Upper panels: representative images of wild-type neurons plated on confluent wild-type or Kidins220−/− astrocyte cultures stained with anti-VGLUT1 (b) or anti-VGAT (c) antibodies at 5, 7, and 10 DIV. Scale bars, 5 µm. Lower panels: quantification of the density of VGLUT1 and VGAT-positive boutons under the various experimental conditions. *p < 0.05, unpaired Student’s t test (n = 20 wild-type and Kidins220−/− cells from five independent cultures). Values are expressed as means ± S.E.M. in all panels
Article Snippet: Antibodies The following primary antibodies were used: rabbit polyclonal anti-Kidins220 (GSC16, #AB34790, Abcam, Cambridge, UK), rabbit monoclonal anti-GAPDH (14C10, #2118, Cell signaling, Leiden, The Netherlands), rabbit polyclonal anti-active caspase 3 (#AF835, R&D Systems, Minneapolis, MN, USA), rabbit anti-β tubulin III (#T2200, Sigma-Aldrich, Milan, Italy),
Techniques: Expressing, Staining